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Helicon focus manual pdf3/6/2023 ![]() The resulting image can even become useless blurring-particularly in fluorescence applications. Deviating from this factory setting creates (for various optical reasons) SA whereby image detail is never properly focused or captured. Manufacturers claim to correct their biological or special industrial objectives for use with a defined cover glass thickness with the preparation just beneath it. This is all due to the fact that the relative position of the objective’s focus is determined by the thickness of the cover glass (or lack thereof) and the medium in which the subject is immersed. Even the finest optics can be thrown out of correction when cover glasses, thick or highly-refractive media are used. ![]() This is the single most overlooked reason for poor microscope imagery. (In SA, an optical system cannot focus all the light at a common plane resulting in a fogging of image details). In biological and high-tech industrial applications, this means that it can be used to diminish or correct the problem of preparation induced spherical aberration (SA). This is more significant than may at first be thought.īeyond its simpler industrial and general uses, InFocus is a significant breakthrough wherever cover glasses must be used. ![]() True optical focusing means that a microscope equipped with InFocus can be deliberately defocused so that a new position can be set for the relative working distance of microscope object to objective. Whether you use InFocus as a self-contained microscope in a breadboard setup-or attached to a microscope stand-it provides added degrees of convenience and perfection. But where the simpler InfiniTubes impart minor focus adjustment, InFocus imparts true optical focusing. InFocus can be thought of as the “ultimate” InfiniTube when infinity-corrected objectives are used with it. InFocus is a unique optical device-in fact, a system-that allows you to realize the full potentials of optical microscopy.
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